Equipment
We provide cutting-edge equipment to help research communities accelerate their research.
Analyzers
Please note we do have an assortment of extra mirrors and filters which can be exchanged, thereby allowing the detection of other fluorochromes.
▸ BD FACS Canto II (3-laser-blue (4 parameter), Red (2
parameter), and Violet (2 parameter))
| Laser specifications | |||
|---|---|---|---|
| Manufacturer | Model | Wavelength (nm) | Power (mW) |
| Coherent | Sapphire 488-20 | 488 | 20 |
| JDS Uniphase | 1144-P | 633 | 17 |
| Point Source | iFlex2000-P-1-404-0.65-30- NP | 405 | 30 |
| Signal processing | Fluidics |
|---|---|
| Data Acquisition channels | Sample flow rates |
| Low = 10 ul/min | |
| Medium = 60 ul/min | |
| High = 120 ul/min |
| Emission optics 488-nm laser | Emission optics 633-nm laser | Emission optics 405-nm laser |
|---|---|---|
| Wavelength ranges detected | Wavelength ranges detected | Wavelength ranges detected |
| 780/60 nm (PE-Cy7) | 780/60 nm (APC-Cy7) | 525/50 nm (AmCyan) |
| 670LP nm (PERCP-Cy5.5, Per CP) | 660/20 nm (APC) | 440/40 nm (Pacific Blue) |
| 585/42 nm (PE) | ||
| 530/30 nm (Fitc) |
▸ Beckman Cytoflex LX (6 lasers, 21 parameters)
| Laser Color/Wavelength | Parameters |
|---|---|
| UV | 3 |
| Violet | 5 |
| Blue | 3 |
| Yellow | 5 |
| Red | 3 |
| IR | 2 |
* Also can run 96 well Plate.
Cell Sorters
▸ BD FACS ARIA III (3-laser-Blue (5 parameter), Red (3
parameter), and Violet (3 parameter))
| Laser specifications | |||
|---|---|---|---|
| Manufacturer | Model | Wavelength (nm) | Power (mW) |
| Coherent | Sapphire | 488 | 20 |
| JDS Uniphase | 1344P | 633 | 17 |
| Coherent | Radius | 405 | 25 |
| Signal processing |
|---|
| Data Acquisition channels |
| Emission optics 488-nm laser | Emission optics 633-nm laser | Emission optics 405-nm laser |
|---|---|---|
| Wavelength ranges detected | Wavelength ranges detected | Wavelength ranges detected |
| 780/60 nm (PE-Cy7) | 780/60 nm (APC-Cy7) | 525/50 nm (AmCyan) |
| 695/40 nm (PERCP-Cy5.5) | 660/20 nm (APC) | 440/40 nm (Pacific Blue) |
| 575/26 (PE,PI) | ||
| 530/30 nm (Fitc) |
* Access to UHN personnel is 24 hours. For external users to the KDT FCF this access may be restricted. Contact flow facility personnel.
Magnetic Cell Separator
AutoMacs Cell Separator:
The AutoMacs Cell Separator provides fast and gentle isolation of any cell type. Cells can be automatically isolated from PBMCs, dissociated tissues or directly from whole blood or bone marrow.
The AutoMacs Cell Separator provides fast and gentle isolation of any cell type. Cells can be automatically isolated from PBMCs, dissociated tissues or directly from whole blood or bone marrow.
