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Krembil Discovery Tower Flow Cytometry Core Facility

Providing instrumentation and expertise to the UHN Investigators and the surrounding research community

Get Started

Welcome to the UHN Research
Krembil Research Institute (KDT) Flow Cytometry Core Facility

We are open to all UHN Investigators and External Client.

New personnel will require training for the instruments they wish to use, and once training has been completed they will be provided security access to the facility.

Equipment

We provide cutting-edge equipment to help research communities accelerate their research.

Analyzers

Please note we do have an assortment of extra mirrors and filters which can be exchanged, thereby allowing the detection of other fluorochromes.

▸ BD FACS Canto II (3-laser-blue (4 parameter), Red (2 parameter), and Violet (2 parameter))
Laser specifications
Manufacturer Model Wavelength (nm) Power (mW)
Coherent Sapphire 488-20 488 20
JDS Uniphase 1144-P 633 17
Point Source iFlex2000-P-1-404-0.65-30- NP 405 30
Signal processing Fluidics
Data Acquisition channels Sample flow rates
Low = 10 ul/min
Medium = 60 ul/min
High = 120 ul/min
Emission optics 488-nm laser Emission optics 633-nm laser Emission optics 405-nm laser
Wavelength ranges detected Wavelength ranges detected Wavelength ranges detected
780/60 nm (PE-Cy7) 780/60 nm (APC-Cy7) 525/50 nm (AmCyan)
670LP nm (PERCP-Cy5.5, Per CP) 660/20 nm (APC) 440/40 nm (Pacific Blue)
585/42 nm (PE)
530/30 nm (Fitc)
▸ Beckman Cytoflex LX (6 lasers, 21 parameters)
Laser Color/Wavelength Parameters
UV 3
Violet 5
Blue 3
Yellow 5
Red 3
IR 2

* Also can run 96 well Plate.

Cell Sorters

▸ BD FACS ARIA III (3-laser-Blue (5 parameter), Red (3 parameter), and Violet (3 parameter))
Laser specifications
Manufacturer Model Wavelength (nm) Power (mW)
Coherent Sapphire 488 20
JDS Uniphase 1344P 633 17
Coherent Radius 405 25
Signal processing
Data Acquisition channels
Emission optics 488-nm laser Emission optics 633-nm laser Emission optics 405-nm laser
Wavelength ranges detected Wavelength ranges detected Wavelength ranges detected
780/60 nm (PE-Cy7) 780/60 nm (APC-Cy7) 525/50 nm (AmCyan)
695/40 nm (PERCP-Cy5.5) 660/20 nm (APC) 440/40 nm (Pacific Blue)
575/26 (PE,PI)
530/30 nm (Fitc)

* Access to UHN personnel is 24 hours. For external users to the KDT FCF this access may be restricted. Contact flow facility personnel.

Magnetic Cell Separator

AutoMacs Cell Separator:
The AutoMacs Cell Separator provides fast and gentle isolation of any cell type. Cells can be automatically isolated from PBMCs, dissociated tissues or directly from whole blood or bone marrow.

Fee Table

Instruments

Unassisted Assisted
Analyzers
Canto II $30/hr $75/hr
Cytoflex LX $40/hr $75/hr
Cell Sorter
Aria III $75/hr Not Available
Magnetic Cell Separator
AutoMacs No fee No fee

Extra Fees

Training
Amount of time required dependant on skill level
A single user designation, and everyone is required to go through the training followed by a test
Follow up 75/hr + instrument time
$75/2hr-3hr
Data Analysis $75/hr
Consults $60/hr
Sorting After Hours $135/hr

External Clients

Wet Lab Research Safety Training $300

* Please note that corporate rates are double.

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